Identification of proteins from tuberculin purified protein derivative (PPD) by LC-MS/MS
نویسندگان
چکیده
منابع مشابه
Identification of proteins from tuberculin purified protein derivative (PPD) by LC-MS/MS.
The tuberculin purified protein derivative (PPD) is a widely used diagnostic antigen for tuberculosis, however it is poorly defined. Most mycobacterial proteins are extensively denatured by the procedure employed in its preparation, which explains previous difficulties in identifying constituents from PPD to characterize their behaviour in B- and T-cell reactions. We here described a proteomics...
متن کاملPurified Protein Derivative--PPD.
A 55-year-old physician had a PPD made as part of a clinical evaluation. Laboratory tests were unremarkable and PPD, read in 48 hours, showed an area of induration of 18 mm and an erythema of 51 mm (Panel A). A 2 mm punch biopsy was done in the center of the induration 96 hours after the injection. Histopathology showed an intense perivascular cuffing with macrophages and lymphocytes and edema ...
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Forty-two patients with neoplastic diseases received intravenous administration of PPD (PPD-V), and tumor markers were monitored between January, 1986 and February, 1987. Tumor markers such as CEA, AFP, SCC, CA125, lAP and polyamines decreased significantly following the PPD-V therapy. In some cases, PPD-V therapy also reduced the progression of clinical manifestation of the disease. We could n...
متن کاملPreparations of Tuberculin Purified Protein Derivative
LANDI, S. (University of Toronto, Toronto, Ontario, Canada), AND H. R. HELD. Physicochemical and biological studies on various preparations of tuberculin purified protein derivative. Appl. Microbiol. 13:132-139. 1965.-Tuberculin purified protein derivative (PPD) has been prepared by seven different precipitation methods from culture filtrate of Mycobacterium tuberculosis var. hominis. It was fo...
متن کاملIdentification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)
Dendritic cells (DC) are known to present exogenous protein Ag effectively to T cells. In this study we sought to identify the proteases that DC employ during antigen processing. The murine epidermal-derived DC line Xs52, when pulsed with PPD, optimally activated the PPD-reactive Th1 clone LNC.2F1 as well as the Th2 clone LNC.4k1, and this activation was completely blocked by chloroquine pretre...
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ژورنال
عنوان ژورنال: Tuberculosis
سال: 2009
ISSN: 1472-9792
DOI: 10.1016/j.tube.2009.07.003